Instead, Valverde has included a number of academy stars in his group for the trip to Milan, with Ansu Fati recalled and Riqui Puig also among the group.
Sergi Roberto and Gerard Pique are also being rested and will stay behind in Barcelona, while Ousmane Dembele, Nelson Semedo, Jordi Alba and Arthur all miss out through injury.
Centre back Ronald Araujo and right back Dani Morer are also among the youthful squad set to feature in the club’s final Champions League group game, with Barca having already secured their spot at the top of the group.
Messi’s absence will come as a huge boost for Inter Milan, who are level on points with Borussia Dortmund and desperately battling to secure their passage to the knockout stages of the competition.
While Antonio Conte’s side host Barcelona, Dortmund face bottom side Slavia Prague in a home match of their own.
BARCELONA’S SQUAD TO FACE INTER MILAN
Marc-Andre Ter Stegen, Ivan Rakitic, Sergio Busquets, Jean-Claude Todibo, Luis Suarez, Neto, Clement Lenglet, Moussa Wague, Antoine Griezmann, Carles Alena, Frenkie de Jong, Arturo Vidal, Samuel Umtiti, Junior Firpo, Inaki Pena, Carles Perez, Riqui Puig, Ronald Araujo, Dani Morer