Jordi Alba, Andres Iniesta and Ivan Rakitic, who started on the bench during defeat to Real Sociedad, are also in the squad and the trio are expected to return to the Barcelona starting XI alongside Suarez on Wednesday night after they made the line-up in the first leg.
Marc-Andre ter Stegen, meanwhile, is also ready to take the place of Claudio Bravo between the sticks as the German is Luis Enrique’s first choice for the custodian role in the Champions League.
As expected, Rafinha Alcantara, Sandro Ramirez, Aleix Vidal, Thomas Vermaelen and Jeremy Mathieu all fail to make the roster due to various injuries.
Ter Stegen, Bravo, Masip, Douglas, Piqué, Rakitic, Sergio, Alves, Arda, Iniesta, Suárez, Messi, Neymar Jr, Mascherano, Bartra, Munir, Jordi Alba, Sergi Roberto and Adriano.
The current Champions League title-holders visit the Vicente Calderon with a slight advantage, having beat Los Colchoneros 2-1 in the first leg at the Nou Camp. Luis Suarez scored two goals to give his side the victory after Fernando Torres opened the scoring for Atletico in the first half, just minutes before being sent off for a second bookable offence.
However, Barcelona arrive to the game under pressure after taking just one point from the last three games and seeing their lead at the top of La Liga cut to just three and four points over Atletico and Real Madrid respectively.
A month ago Barcelona looked destined to win another record-breaking treble, but their sudden loss of form threatens to leave them trophyless this season.